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Focus variation is a method to calculate a sharp image and to measure the depth with an optics with limited depth of field. ==Algorithm== The algorithm work as follows: # at first images with difference focus are captured. This is done by moving the sample or the optics in relation to each other. # then for each position the focus over each plane is calculated # the plane with the best focus is used to get a sharp image. the corresponding depth gives the depth at this position- 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Focus variation」の詳細全文を読む スポンサード リンク
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